Nfatc2^tm1Glm
Targeted Allele Detail

Summary

• Symbol: Nfatc2^tm1Glm
• Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2; targeted mutation 1, Laurie H Glimcher
• MGI ID: MGI:2182740
• Synonyms: c2-, NFATc2-, NF-ATp-mut
• Gene: Nfatc2 Location: Chr2:168318330-168443577 bp, - strand Genetic Position: Chr2, 88.91 cM
• Alliance: Nfatc2^tm1Glm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:32728
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced sequences encoding 47 amino acids of the Rel homology domain. An absence of normal protein in homozygous mutant mice was determined through Western blot analysis. Low levels of reactive polypeptides were present in the cytoplasm, however, no reactivity was observed in the nucleus. RT-PCR and sequence analysis revealed aberrantly spliced in-frame transcripts from which the targeted exon had been excised. An electrophoretic mobility shift assay confirmed that DNA was not binding to the mutant protein. (J:32728)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nfatc2 Mutation: 53 strains or lines available

References

• Original: J:32728 Hodge MR, et al., Hyperproliferation and dysregulation of IL-4 expression in NF-ATp-deficient mice. Immunity. 1996 Apr;4(4):397-405
• All: 41 reference(s)