Nfatc2^tm1Rao
Targeted Allele Detail

Summary

• Symbol: Nfatc2^tm1Rao
• Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2; targeted mutation 1, Anjana Rao
• MGI ID: MGI:2182743
• Synonyms: NFAT1^-, NFATC2-
• Gene: Nfatc2 Location: Chr2:168318330-168443577 bp, - strand Genetic Position: Chr2, 88.91 cM
• Alliance: Nfatc2^tm1Rao page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:33105
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: An amino terminal exon, encoding residues conserved in DNA-binding domains, was disrutped by the insertion of a PGK-neo cassette. RT-PCR analysis indicated aberrant splicing in which the targeted exon had been excised, resulting in a frameshift mutation that introduced a nonsense mutation at amino acid 438. Protein encoded by the mutant transcript was undetected by several antibodies in extracts from homozygous mutant mice. A DNA-binding assay showed reduced activity in cytosolic extracts of homozygous mutant T cells (the residual activity was attributed to the presence of protein encoded by Nfatc1). (J:33105)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Nfatc2 Mutation: 53 strains or lines available

References

• Original: J:33105 Xanthoudakis S, et al., An enhanced immune response in mice lacking the transcription factor NFAT1. Science. 1996 May 10;272(5263):892-5
• All: 35 reference(s)