Rnasel^tm1Slvm
Targeted Allele Detail

Summary

• Symbol: Rnasel^tm1Slvm
• Name: ribonuclease L (2', 5'-oligoisoadenylate synthetase-dependent); targeted mutation 1, Robert H Silverman
• MGI ID: MGI:2182763
• Synonyms: RL^-, RNase L^-
• Gene: Rnasel Location: Chr1:153625172-153639967 bp, + strand Genetic Position: Chr1, 65.43 cM, cytoband G2
• Alliance: Rnasel^tm1Slvm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:44292
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Exon 1, encoding the activator-binding region, was disrupted at codon 100 by the insertion of a neomycin selection cassette. The absence of normal protein in homozygous mutant mice was determined via Western blot analysis of MEF protein extracts and by binding assays on extracts from various organs. No ribonuclease activity was detected in MEF via Northern blot analysis. (J:44292)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rnasel Mutation: 26 strains or lines available

References

• Original: J:44292 Zhou A, et al., Interferon action and apoptosis are defective in mice devoid of 2',5'-oligoadenylate-dependent RNase L. EMBO J. 1997 Nov 3;16(21):6355-63
• All: 31 reference(s)