Bhlha15^tm1Skz
Targeted Allele Detail

Summary

• Symbol: Bhlha15^tm1Skz
• Name: basic helix-loop-helix family, member a15; targeted mutation 1, Stephen F Konieczny
• MGI ID: MGI:2182768
• Synonyms: Bhlhb8^-, Bhlhb8^lacZ, Mist1^-, Mist1^KO, Mist1^LacZ
• Gene: Bhlha15 Location: Chr5:144127104-144131259 bp, + strand Genetic Position: Chr5, 83.05 cM, cytoband G2-G3
• Alliance: Bhlha15^tm1Skz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:72798
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The entire coding region of the gene was replaced with a lacZ-neo cassette via homologous recombination. Absence of gene expression in homozygous mutant animals was confirmed by immunohistochemical analysis, Western blot and RT-PCR. (J:72798)

Expression

In Mice Carrying this Mutation:	15 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Bhlha15 Mutation: 15 strains or lines available

References

• Original: J:72798 Pin CL, et al., The bHLH transcription factor Mist1 is required to maintain exocrine pancreas cell organization and acinar cell identity. J Cell Biol. 2001 Nov 12;155(4):519-30
• All: 23 reference(s)