Nfatc2^tm1Srf
Targeted Allele Detail

Summary

• Symbol: Nfatc2^tm1Srf
• Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2; targeted mutation 1, Edgar Serfling
• MGI ID: MGI:2182770
• Synonyms: NF-ATp^-
• Gene: Nfatc2 Location: Chr2:168318330-168443577 bp, - strand Genetic Position: Chr2, 88.91 cM
• Alliance: Nfatc2^tm1Srf page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:45252
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 470 base pairs of exon 2 and approximately 400 base pairs of intron 2 were replaced with a neomycin selection cassette. The insertion of the neo gene removed a splice site and introduced stop codons into all three reading frames. Western blot analysis of splenocyte nuclear protein extracts confirmed the absence of normal encoded protein in homozygous mutant mice. (J:45252, J:110686, J:150931)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nfatc2 Mutation: 53 strains or lines available

References

• Original: J:45252 Heyer J, et al., Inefficient termination of antigen responses in NF-ATp-deficient mice. Immunobiology. 1997 Dec;198(1-3):162-9
• All: 6 reference(s)