H1f0^tm1Ais
Targeted Allele Detail

Summary

• Symbol: H1f0^tm1Ais
• Name: H1.0 linker histone; targeted mutation 1, Art I Skoultchi
• MGI ID: MGI:2182779
• Synonyms: H1^0-/-
• Gene: H1f0 Location: Chr15:78912650-78914704 bp, + strand Genetic Position: Chr15, 37.7 cM
• Alliance: H1f0^tm1Ais page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:26848
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The majority of the gene (from 5' of the ATG start site to codon 170 comprising 90% of the coding sequence) was replaced with a neomycin resistance cassette via homologous recombination. Absence of gene expression in homozygous mutant animals was confirmed by Western blot analysis of histone extracts. (J:26848)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any H1f0 Mutation: 13 strains or lines available

References

• Original: J:26848 Sirotkin AM, et al., Mice develop normally without the H1(0) linker histone. Proc Natl Acad Sci U S A. 1995 Jul 3;92(14):6434-8
• All: 5 reference(s)