Ppef2^tm1Nat
Targeted Allele Detail

Summary

• Symbol: Ppef2^tm1Nat
• Name: protein phosphatase, EF hand calcium-binding domain 2; targeted mutation 1, Jeremy Nathans
• MGI ID: MGI:2182812
• Synonyms: PPEF-2^-
• Gene: Ppef2 Location: Chr5:92374538-92404137 bp, - strand Genetic Position: Chr5, 46.28 cM, cytoband E3
• Alliance: Ppef2^tm1Nat page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:72941
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The last 14 codons of exon 6 and all of exons 7-9 were replaced with a lacZ-neo cassette via homologous recombination and results in an in-frame fusion of the lacZ gene to exon 6. Gene expression was absent in retinas of homozygous mutant animals as determined by Western blot analysis and immunostaining using antibodies directed against the C-terminus of the protein. Beta-galactosidase activity was not present in the mutant animals. (J:72941)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ppef2 Mutation: 33 strains or lines available

References

• Original: J:72941 Ramulu P, et al., Normal Light Response, Photoreceptor Integrity, and Rhodopsin Dephosphorylation in Mice Lacking Both Protein Phosphatases with EF Hands (PPEF-1 and PPEF-2). Mol Cell Biol. 2001 Dec;21(24):8605-14
• All: 1 reference(s)