Epb42^tm1Llp
Targeted Allele Detail

Summary

• Symbol: Epb42^tm1Llp
• Name: erythrocyte membrane protein band 4.2; targeted mutation 1, Luanne L Peters
• MGI ID: MGI:2182816
• Synonyms: 4.2^-
• Gene: Epb42 Location: Chr2:120848372-120867358 bp, - strand Genetic Position: Chr2, 60.37 cM
• Alliance: Epb42^tm1Llp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:67412
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Sequence from exons 4 through 8 was replaced with a neomycin selection cassette. Northern blot analysis of total reticulocyte RNA and RNase protection assays of nonerythroid tissue samples did not detect message encoded by the targeted allele. Protein was determined to be absent in erythroid cells from homozygous mutant mice via Western blot analysis and Coomassie blue staining. (J:67412)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Epb42 Mutation: 41 strains or lines available

References

• Original: J:67412 Peters LL, et al., Mild spherocytosis and altered red cell ion transport in protein 4. 2-null mice. J Clin Invest. 1999 Jun;103(11):1527-37
• All: 6 reference(s)