Mark2^tm1Hpw
Targeted Allele Detail

Summary

• Symbol: Mark2^tm1Hpw
• Name: MAP/microtubule affinity regulating kinase 2; targeted mutation 1, Helen Piwnica-Worms
• MGI ID: MGI:2182819
• Synonyms: Enk^-, Mark2/EMK^-, Par-1b^-
• Gene: Mark2 Location: Chr19:7252761-7319222 bp, - strand Genetic Position: Chr19, 5.32 cM, cytoband B
• Alliance: Mark2^tm1Hpw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:68830
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The insertion of a neomycin selection cassette deleted sequence from exons 2, 3, and 4. The replaced region encoded residues of the glycine-rich motif involved in MgATP-binding. Western blot analysis of extracts from fibroblasts showed an absence of all known isoforms produced from the targeted allele in homozygous mutant embryos. Immunoprecipitates of tissue extracts derived from homozygous mutant mice failed to phosphorylate GST-Cdc25C in vitro, incdicating ablation of kinase activity. (J:68830)

Expression

In Structures Affected by this Mutation:

12 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mark2 Mutation: 109 strains or lines available

References

• Original: J:68830 Hurov JB, et al., Immune system dysfunction and autoimmune disease in mice lacking Emk (Par-1) protein kinase. Mol Cell Biol. 2001 May;21(9):3206-19
• All: 7 reference(s)