F2rl3^tm1.1Cgh
Targeted Allele Detail

Summary

• Symbol: F2rl3^tm1.1Cgh
• Name: F2R like thrombin or trypsin receptor 3; targeted mutation 1.1, Shaun R Coughlin
• MGI ID: MGI:2182919
• Gene: F2rl3 Location: Chr8:73488508-73490502 bp, + strand Genetic Position: Chr8, 35.08 cM, cytoband B3.3
• Alliance: F2rl3^tm1.1Cgh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:71391
• Parent Cell Line: RF8 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Mice bearing this allele were generated by crossing F2rl3^tm1Cgh mice with mice constitutively expressing cre recombinase. PCR and Southern blot analysis showed that the expression of cre resulted in the excision of the floxed neo gene, leaving the lacZ transgene at the start codon of the endogenous gene. Comparisons of the results of beta-galactosidase staining of heterozygotes and in situ hybridization of wild-type mice, indicated that expression of the lacZ gene was controlled by the endogenous promoter. (J:71391)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any F2rl3 Mutation: 21 strains or lines available

References

• Original: J:71391 Sambrano GR, et al., Role of thrombin signalling in platelets in haemostasis and thrombosis. Nature. 2001 Sep 6;413(6851):74-8
• All: 22 reference(s)