Slc22a1^tm1Ahs
Targeted Allele Detail

Summary

• Symbol: Slc22a1^tm1Ahs
• Name: solute carrier family 22 (organic cation transporter), member 1; targeted mutation 1, Alfred H Schinkel
• MGI ID: MGI:2182947
• Synonyms: Oct1^-
• Gene: Slc22a1 Location: Chr17:12867756-12894716 bp, - strand Genetic Position: Chr17, 8.63 cM
• Alliance: Slc22a1^tm1Ahs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:70604
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 7 encoding putative transmembrane domains 7-9 and flanking intronic sequences were replaced with a PGK-hygro cassette in the reverse orientation via homologous recombination. Northern blot analysis did not detect gene expression in liver or kidney of homozygous mutant animals. However, RNase protection assay using a probe to nucleotides 447 to 691 lying outside the deleted region detected extremely low levels of transcript, which may represent a truncated and unstable message. Western blot analysis of homozygous mutant liver and kidney samples using a polyclonal antibody probe recognizing the C-terminal region of rat and mouse protein further verified the absence of gene expression. (J:70604)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc22a1 Mutation: 31 strains or lines available

References

• Original: J:70604 Jonker JW, et al., Reduced hepatic uptake and intestinal excretion of organic cations in mice with a targeted disruption of the organic cation transporter 1 (Oct1 [Slc22a1]) gene. Mol Cell Biol. 2001 Aug;21(16):5471-7
• All: 8 reference(s)