Elk3^tm1Wlyk
Targeted Allele Detail

Summary

• Symbol: Elk3^tm1Wlyk
• Name: ELK3, member of ETS oncogene family; targeted mutation 1, Bohdan Wasylyk
• MGI ID: MGI:2182950
• Synonyms: net^delta, Net^mutant
• Gene: Elk3 Location: Chr10:93083276-93146997 bp, - strand Genetic Position: Chr10, 48.04 cM, cytoband C-D1
• Alliance: Elk3^tm1Wlyk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:71816
• Parent Cell Line: D4 (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 2, which encodes the first 69 amino acids (including the initiator methionine), and surrounding intronic sequence were replaced with a neomycin selection cassette. RT-PCR and sequence analysis showed the presence of an aberrantly spliced transcript in which exon 1 had joined exon 3. The aberrant transcript was shown by Western blot analysis to produce a 37kDa protein via an in-frame translational start site at nucleotide 571 (aa 93). The mutant protein lacks the Ets DNA-binding domain. (J:71816)

Disease models

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Elk3 Mutation: 20 strains or lines available

References

• Original: J:71816 Ayadi A, et al., Net-targeted mutant mice develop a vascular phenotype and up-regulate egr-1. EMBO J. 2001 Sep 17;20(18):5139-52
• All: 6 reference(s)