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Runx1t1tm1Fc
Targeted Allele Detail
Summary
Symbol: Runx1t1tm1Fc
Name: RUNX1 translocation partner 1; targeted mutation 1, Franco Calabi
MGI ID: MGI:2183012
Synonyms: MTG8Ex2/lacZ
Gene: Runx1t1  Location: Chr4:13743436-13893649 bp, + strand  Genetic Position: Chr4, 5.88 cM, cytoband A
Alliance: Runx1t1tm1Fc page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:70590
Parent Cell Line:  CCB (ES Cell)
Strain of Origin:  129S/SvEv-Gpi1c
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Insertion
 
Mutation detailsThe gene was disrupted by an in-frame insertion of a lacZ-neo cassette into the 3' end of exon 2, which is the common splice acceptor site for several upstream exons. RNase protection assay using a cDNA probe showed the absence the exon 2-4 splice product in brains of homozygous mutants, but an alternative splice product upstream of exon 3 was increased. Western blot of brain extracts from homozygous mutants showed an absence of the 75 kDa and 90 kDa products, presence of the 200 kDa product, and an increase in the 55 kDa product. (J:70590)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 7 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Runx1t1 Mutation:  37 strains or lines available
References
Original:  J:70590 Calabi F, et al., Gene targeting reveals a crucial role for mtg8 in the gut. Mol Cell Biol. 2001 Aug;21(16):5658-66
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory