Nfatc4^tm1Grc
Targeted Allele Detail

Summary

• Symbol: Nfatc4^tm1Grc
• Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 4; targeted mutation 1, Gerald R Crabtree
• MGI ID: MGI:2183093
• Synonyms: c4-, NFATc4^-
• Gene: Nfatc4 Location: Chr14:56062252-56071400 bp, + strand Genetic Position: Chr14, 28.19 cM, cytoband C1
• Alliance: Nfatc4^tm1Grc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:70266
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 1-2 and part of exon 3 were replaced with neomycin resistance gene via homologous recombination. This region of the gene encodes amino acids 1-413 and contains the translation start site, a regulatory domain that controls calmodulin-dependent cytoplasmic-to-nuclear translocation, and part of the DNA-binding domain. Western blot and RNase protection assay of homozygous mutant embryos verified the absence of gene expression. (J:70266)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nfatc4 Mutation: 38 strains or lines available

References

• Original: J:70266 Graef IA, et al., Signals transduced by Ca(2+)/calcineurin and NFATc3/c4 pattern the developing vasculature. Cell. 2001 Jun 29;105(7):863-75
• All: 10 reference(s)