Thra^tm2Jas
Targeted Allele Detail

Summary

• Symbol: Thra^tm2Jas
• Name: thyroid hormone receptor alpha; targeted mutation 2, Jacques Samarut
• MGI ID: MGI:2183135
• Synonyms: TRalpha⁰
• Gene: Thra Location: Chr11:98631539-98659832 bp, + strand Genetic Position: Chr11, 62.58 cM, cytoband D-E
• Alliance: Thra^tm2Jas page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:70007
• Parent Cell Line: ENS (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 5 through 7 were replaced with a cassette comprised of lacZ and neo. The targeting vector was designed to abolish transcription of the four known isoforms produced from this locus. The deleted region encoded the DNA-binding domain found in the two larger isoforms and the internal promoter in intron 7 that drives the transcription of the two truncated isoforms. Northern blot and RT-PCR analyses of intestinal RNA showed the absence of all four transcripts in homozygous mutant mice. RNA protection assays revealed the occasional presence of the truncated transcripts resulting from sporadic readthrough of the stop codons contained within the inserted cassette. (J:70007)

Expression

In Mice Carrying this Mutation:	18 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	17 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Thra Mutation: 40 strains or lines available

References

• Original: J:70007 Gauthier K, et al., Genetic analysis reveals different functions for the products of the thyroid hormone receptor alpha locus. Mol Cell Biol. 2001 Jul;21(14):4748-60
• All: 34 reference(s)