Gabra2^tm1.1Uru
Targeted Allele Detail

Summary

• Symbol: Gabra2^tm1.1Uru
• Name: gamma-aminobutyric acid type A receptor subunit alpha 2; targeted mutation 1.1, Uwe Rudolph
• MGI ID: MGI:2183163
• Synonyms: alpha2(H101R), alpha2^R, mutant allele 1
• Gene: Gabra2 Location: Chr5:71115735-71253192 bp, - strand Genetic Position: Chr5, 37.59 cM
• Alliance: Gabra2^tm1.1Uru page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:65100
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: This allele was generated by breeding mice carrying Gabra2^tm1Uru with transgenic mice expressing cre recombinase via the EIIa promoter. The floxed neomycin cassette was excised from intron 3, while the point mutation in codon 101 that results in a histidine to arginine substitution remained intact. Western blot analysis showed that the substitution did not affect expression of the protein. Autoradiographs of parasagittal brain sections showed novel diazepam-insensitve sites in regions where the endogenous protein is expressed, indicating the disruption of the drug-binding site. (J:65100)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gabra2 Mutation: 36 strains or lines available

References

• Original: J:65100 Low K, et al., Molecular and neuronal substrate for the selective attenuation of anxiety. Science. 2000 Oct 6;290(5489):131-4
• All: 15 reference(s)