Gabra3^tm1.1Uru
Targeted Allele Detail

Summary

• Symbol: Gabra3^tm1.1Uru
• Name: gamma-aminobutyric acid type A receptor subunit alpha 3; targeted mutation 1.1, Uwe Rudolph
• MGI ID: MGI:2183168
• Synonyms: alpha3(H126R), mutant allele 2
• Gene: Gabra3 Location: ChrX:71476287-71700454 bp, - strand Genetic Position: ChrX, 37.24 cM
• Alliance: Gabra3^tm1.1Uru page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:65100
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Single point mutation
• Mutation details: This allele was generated by breeding mice carrying Gabra3^tm1Uru with transgenic mice expressing cre recombinase via the EIIa promoter. The floxed neomycin cassette was excised from intron 3, while the point mutation in codon 126 that results in a histidine to arginine substitution remained intact. Western blot analysis showed that the substitution did not affect expression of the protein. Autoradiographs of parasagittal brain sections showed novel diazepam-insensitve sites in regions where the endogenous protein is expressed, indicating the disruption of the drug-binding site. (J:65100)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gabra3 Mutation: 8 strains or lines available

References

• Original: J:65100 Low K, et al., Molecular and neuronal substrate for the selective attenuation of anxiety. Science. 2000 Oct 6;290(5489):131-4
• All: 12 reference(s)