Ucp3^tm1Rei
Targeted Allele Detail

Summary

• Symbol: Ucp3^tm1Rei
• Name: uncoupling protein 3 (mitochondrial, proton carrier); targeted mutation 1, Marc L Reitman
• MGI ID: MGI:2183202
• Synonyms: Ucp3 (-)
• Gene: Ucp3 Location: Chr7:100122198-100135639 bp, + strand Genetic Position: Chr7, 54.36 cM
• Alliance: Ucp3^tm1Rei page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:62312
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 4.0 kb of sequence, including the first coding exon (exon 2), was deleted and replaced with a neomycin selection cassette. Northern blot and RT-PCR analyses detected aberrantly spliced transcript, in which exon 1 had spliced directly to exon 3, in homozygous mutant mice. Protein encoded from the aberrant transcript was undetected via Western blot analysis. (J:62312)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Ucp3^tm1Rei

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ucp3 Mutation: 6 strains or lines available

References

• Original: J:62312 Gong DW, et al., Lack of obesity and normal response to fasting and thyroid hormone in mice lacking uncoupling protein-3. J Biol Chem. 2000 May 26;275(21):16251-7
• All: 19 reference(s)