Smpd2^tm1Wst
Targeted Allele Detail

Summary

• Symbol: Smpd2^tm1Wst
• Name: sphingomyelin phosphodiesterase 2, neutral; targeted mutation 1, Wilhelm Stoffel
• MGI ID: MGI:2183203
• Synonyms: nsmase 1^-/-
• Gene: Smpd2 Location: Chr10:41363168-41366410 bp, - strand Genetic Position: Chr10, 22.42 cM
• Alliance: Smpd2^tm1Wst page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76664
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2-8 were replaced with a PGK-neo derived cassette via homologous recombination. Northern blot analysis verified the absence of gene expression in kidney, liver, and brain of homozygous mutant animals. RT-PCR analysis detected a low level of mutant transcripts resulting from the splicing of the neo gene to exon 9. Western blot analysis did not detect protein product in membrane fractions of kidney, liver, and brain from homozygous mutant animals. (J:76664)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Smpd2 Mutation: 19 strains or lines available

References

• Original: J:76664 Zumbansen M, et al., Neutral sphingomyelinase 1 deficiency in the mouse causes no lipid storage disease. Mol Cell Biol. 2002 Jun;22(11):3633-8
• All: 3 reference(s)