Pgr^tm1Omc
Targeted Allele Detail

Summary

• Symbol: Pgr^tm1Omc
• Name: progesterone receptor; targeted mutation 1, Orla M Conneely
• MGI ID: MGI:2183208
• Synonyms: PRAKO^-
• Gene: Pgr Location: Chr9:8899834-8968612 bp, + strand Genetic Position: Chr9, 2.46 cM
• Alliance: Pgr^tm1Omc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77661
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: To eliminate translation of isoform A, without abrogating the translation of isoform B, the translational start site at codon 166, which is specific to isoform A, was modified to encode alanine. For selection, a silent mutation was introduced resulting in the generation of a novel NheI restriction site and a floxed neomycin selection cassette was inserted into intron 2. Recombinant ES cells were transfected with plasmids expressing cre recombinase, resulting in the excision of the neomycin cassette. Immunoblot analysis of uterine extracts from estrogen treated homozygous mutant mice showed an absence of isoform A. Isoform B protein levels were unaffected. (J:77661)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Pgr Mutation: 75 strains or lines available

References

• Original: J:77661 Mulac-Jericevic B, et al., Subgroup of reproductive functions of progesterone mediated by progesterone receptor-B isoform. Science. 2000 Sep 8;289(5485):1751-4
• All: 6 reference(s)