Rasgrf2^tm1Esn
Targeted Allele Detail

Summary

• Symbol: Rasgrf2^tm1Esn
• Name: RAS protein-specific guanine nucleotide-releasing factor 2; targeted mutation 1, Eugenio Santos
• MGI ID: MGI:2183211
• Synonyms: grf2^-, Rasgrf2^-
• Gene: Rasgrf2 Location: Chr13:92028519-92268164 bp, - strand Genetic Position: Chr13, 47.43 cM, cytoband C3-D1
• Alliance: Rasgrf2^tm1Esn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:75928
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A portion of the CDC25-H domain, a highly conserved region of the gene, from amino acids 1005-1070 was replaced with a PGK-neo cassette via homologous recombination. Absence of gene expression in homozygous mutant animals was confirmed by RT-PCR analysis of brain RNA and absence of protein product was demonstrated by Western blot of brain and lung extracts using an antibody directed against a region of the protein upstream of the disruption. (J:75928)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rasgrf2 Mutation: 73 strains or lines available

References

• Original: J:75928 Fernandez-Medarde A, et al., Targeted disruption of Ras-Grf2 shows its dispensability for mouse growth and development. Mol Cell Biol. 2002 Apr;22(8):2498-504
• All: 8 reference(s)