Gfap^tm1Ito
Targeted Allele Detail

Summary

• Symbol: Gfap^tm1Ito
• Name: glial fibrillary acidic protein; targeted mutation 1, Shigeyoshi Itohara
• MGI ID: MGI:2183216
• Synonyms: Gfap^-
• Gene: Gfap Location: Chr11:102778162-102791368 bp, - strand Genetic Position: Chr11, 66.48 cM, cytoband D
• Alliance: Gfap^tm1Ito page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:22502
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A cassette, containing a promoterless lacZ and neo, was inserted immediately downstream of the endogenous start codon. Western blot analysis of protein extracts obtained from cerebrum, cerebellum, and spinal cord tissue showed an absence of encoded protein in homozygous mutant mice. The expression pattern of beta-galactosidase showed a strong correlation to that of the endogenous gene. (J:22502)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gfap Mutation: 48 strains or lines available

References

• Original: J:22502 Gomi H, et al., Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prions. Neuron. 1995 Jan;14(1):29-41
• All: 9 reference(s)