Tnfrsf11b^tm1Wss
Targeted Allele Detail

Summary

• Symbol: Tnfrsf11b^tm1Wss
• Name: tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin); targeted mutation 1, W Scott Simonet
• MGI ID: MGI:2183229
• Synonyms: OPG^-
• Gene: Tnfrsf11b Location: Chr15:54114014-54141700 bp, - strand Genetic Position: Chr15, 21.15 cM
• Alliance: Tnfrsf11b^tm1Wss page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:47602
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette into exon 2 replaced sequence encoded the first 93 residues of the mature protein. This targeting strategy eliminated the first two cysteine-rich domains, which are essential for function, and introduced an in-frame stop codon. Northern blot analysis of total hepatic RNA confirmed the absence of transcript in homozygous mutant mice. (J:47602)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tnfrsf11b Mutation: 31 strains or lines available

References

• Original: J:47602 Bucay N, et al., osteoprotegerin-deficient mice develop early onset osteoporosis and arterial calcification. Genes Dev. 1998 May 1;12(9):1260-8
• All: 7 reference(s)