Siah1a^tm1Ddlb
Targeted Allele Detail

Summary

• Symbol: Siah1a^tm1Ddlb
• Name: siah E3 ubiquitin protein ligase 1A; targeted mutation 1, David D L Bowtell
• MGI ID: MGI:2183245
• Synonyms: Siah1a-
• Gene: Siah1a Location: Chr8:87450635-87472562 bp, - strand Genetic Position: Chr8, 42.1 cM
• Alliance: Siah1a^tm1Ddlb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:75489
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Most of the coding region of the gene was replaced with a PGK-neo cassette facing the opposite orientation via homologous recombination leaving the first 22 codons intact. Northern blot analysis using a probe to the coding region showed decreased expression in the brain and almost no expression in testes of homozygous mutant animals. The low level of transcript detected is presumed to be Siah1b, which also hybridizes to the probe. (J:75489)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Siah1a Mutation: 15 strains or lines available

References

• Original: J:75489 Dickins RA, et al., The ubiquitin ligase component Siah1a is required for completion of meiosis I in male mice. Mol Cell Biol. 2002 Apr;22(7):2294-303
• All: 7 reference(s)