Nefh^tm1Dwc
Targeted Allele Detail

Summary

• Symbol: Nefh^tm1Dwc
• Name: neurofilament, heavy polypeptide; targeted mutation 1, Don W Cleveland
• MGI ID: MGI:2183246
• Synonyms: hko
• Gene: Nefh Location: Chr11:4888754-4898064 bp, - strand Genetic Position: Chr11, 3.12 cM, cytoband A1-A5
• Alliance: Nefh^tm1Dwc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77654
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The insertion of a neomycin selection cassette deleted 1.6kb of sequence containing the proximal promoter and 34 codons, including the initiator methionine codon. Transcript was undetected in homozygous mutant mice by Northern blot analysis of RNA obtained from the brain and the spinal cord. The absence of protein was confirmed via Western blot analysis using several antibodies, capable of detecting either phosphorylated or unphosphorylated protein. (J:77654)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nefh Mutation: 47 strains or lines available

References

• Original: J:77654 Rao MV, et al., Neurofilament-dependent radial growth of motor axons and axonal organization of neurofilaments does not require the neurofilament heavy subunit (NF-H) or its phosphorylation. J Cell Biol. 1998 Oct 5;143(1):171-81
• All: 2 reference(s)