Clcn2^tm1Tjj
Targeted Allele Detail

Summary

• Symbol: Clcn2^tm1Tjj
• Name: chloride channel, voltage-sensitive 2; targeted mutation 1, Thomas J Jentsch
• MGI ID: MGI:2183261
• Synonyms: Clcn2^-
• Gene: Clcn2 Location: Chr16:20521714-20536496 bp, - strand Genetic Position: Chr16, 12.5 cM
• Alliance: Clcn2^tm1Tjj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:68137
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A portion of exon 3 and all of exons 4 through 7 were replaced with a neomycin selection cassette. The deleted region encoded transmembrane domains 2 through 6. Truncated transcript was detected by Northern blot analysis using 5' and neo probes and undetected using a 3' probe. Protein was shown to be absent in homozygous mutant mice via Western blot analysis. (J:68137)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	15 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Clcn2 Mutation: 40 strains or lines available

References

• Original: J:68137 Bosl MR, et al., Male germ cells and photoreceptors, both dependent on close cell-cell interactions, degenerate upon ClC-2 Cl(-) channel disruption. EMBO J. 2001 Mar 15;20(6):1289-99
• All: 13 reference(s)