Nkx3-2^tm1Tno
Targeted Allele Detail

Summary

• Symbol: Nkx3-2^tm1Tno
• Name: NK3 homeobox 2; targeted mutation 1, Tetsuo Noda
• MGI ID: MGI:2183276
• Synonyms: Bapx1^-, Bapx-1^LacZ
• Gene: Nkx3-2 Location: Chr5:41918826-41921563 bp, - strand Genetic Position: Chr5, 22.58 cM
• Alliance: Nkx3-2^tm1Tno page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77612
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The entire coding region, spanning exons 1 and 2, was replaced with a cassette containing an NLS-lacZ gene and a floxed neo gene. The cassette was inserted at the translational start codon, which enabled expression of beta-galactosidase from the endogenous promoter. Northern blot analysis of total RNA obtained from homozygous mutant embryos showed a lack of transcript. (J:77612)

Expression

In Structures Affected by this Mutation:

19 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nkx3-2 Mutation: 21 strains or lines available

References

• Original: J:77612 Akazawa H, et al., Targeted disruption of the homeobox transcription factor Bapx1 results in lethal skeletal dysplasia with asplenia and gastroduodenal malformation. Genes Cells. 2000 Jun;5(6):499-513
• All: 1 reference(s)