Pfn1^tm1Wit
Targeted Allele Detail

Summary

• Symbol: Pfn1^tm1Wit
• Name: profilin 1; targeted mutation 1, Walter Witke
• MGI ID: MGI:2183296
• Synonyms: pfn1^ko
• Gene: Pfn1 Location: Chr11:70542676-70545470 bp, - strand Genetic Position: Chr11, 43.21 cM, cytoband A-D
• Alliance: Pfn1^tm1Wit page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77643
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Disruption of the locus was achieved by the insertion of a neomycin selection cassette at the start codon. Western blot analysis of thymocyte lysates showed a reduction in protein levels of approximately 50% in heterozygous mutant mice. (J:77643)

Expression

In Mice Carrying this Mutation:

1 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pfn1 Mutation: 12 strains or lines available

References

• Original: J:77643 Witke W, et al., Profilin I is essential for cell survival and cell division in early mouse development. Proc Natl Acad Sci U S A. 2001 Mar 27;98(7):3832-6
• All: 4 reference(s)