Clcn3^tm1Tjj
Targeted Allele Detail

Summary

• Symbol: Clcn3^tm1Tjj
• Name: chloride channel, voltage-sensitive 3; targeted mutation 1, Thomas J Jentsch
• MGI ID: MGI:2183297
• Synonyms: ClC3^-, Clcn3^-
• Gene: Clcn3 Location: Chr8:61363423-61436334 bp, - strand Genetic Position: Chr8, 30.9 cM
• Alliance: Clcn3^tm1Tjj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77635
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 3, encoding the first transmembrane domain, and surrounding intronic sequence was replaced with a neomycin selection cassette inserted by homologous recombination. The absence of transcript in homozygous mutant mice was determined by an RNAse protection assay of brain RNA. Western blot analysis, using a specific antibody directed against the amino terminal region, indicated a lack of encoded protein. (J:77635)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Clcn3 Mutation: 120 strains or lines available

References

• Original: J:77635 Stobrawa SM, et al., Disruption of ClC-3, a chloride channel expressed on synaptic vesicles, leads to a loss of the hippocampus. Neuron. 2001 Jan;29(1):185-96
• All: 10 reference(s)