Cebpatm1.1(Cebpb)Ylee
Targeted Allele Detail
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Symbol: |
Cebpatm1.1(Cebpb)Ylee |
Name: |
CCAAT/enhancer binding protein alpha; targeted mutation 1.1, Ying-Hue Lee |
MGI ID: |
MGI:2183333 |
Synonyms: |
C/ebpa-alphabeta |
Gene: |
Cebpa Location: Chr7:34818718-34821353 bp, + strand Genetic Position: Chr7, 21.02 cM
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Alliance: |
Cebpatm1.1(Cebpb)Ylee page
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Germline Transmission: |
Earliest citation of germline transmission:
J:77646
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Parent Cell Line: |
RW-4 (ES Cell)
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Strain of Origin: |
129X1/SvJ
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Allele Type: |
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Targeted (Inserted expressed sequence, Null/knockout) |
Mutation: |
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Insertion
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Cebpatm1.1(Cebpb)Ylee expresses
1 gene
Knock-in expresses:
Organism |
Expressed Gene |
Note |
mouse |
Cebpb (MGI:88373) |
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Mutation details: This allele was generated by breeding mice homozygous for Cebpatm1(Cebpb)Ylee with transgenic mice expressing cre recombinase, driven by the EIIa promoter. Expression of cre resulted in the excision of the floxed neo gene downstream of the stop codon. The Cebpb coding region inserted at the endogenous start codon to replace the coding region of Cebpa remained intact. Northern blot analysis confirmed the absence of Cebpa mRNA and the presence of the Cebpa/Cebpb hybrid mRNA in homozygous mutant mice. Western blot analysis showed that Cebpb protein levels were concomitant with the combined amount of Cebpb and Cebpa/Cebpb transcript until the age of 7 weeks.
(J:77646)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Cebpa Mutation: |
19 strains or lines available
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Original: |
J:77646 Chen SS, et al., C/EBPbeta, when expressed from the C/ebpalpha gene locus, can functionally replace C/EBPalpha in liver but not in adipose tissue. Mol Cell Biol. 2000 Oct;20(19):7292-9 |
All: |
5 reference(s) |
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