Tbp^tm1.1Ees
Targeted Allele Detail

Summary

• Symbol: Tbp^tm1.1Ees
• Name: TATA box binding protein; targeted mutation 1.1, Edward E Schmidt
• MGI ID: MGI:2183359
• Synonyms: tbp^deltaN
• Gene: Tbp Location: Chr17:15720150-15737689 bp, + strand Genetic Position: Chr17, 8.95 cM
• Alliance: Tbp^tm1.1Ees page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77714
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: Animals heterozygous for Tbp^tm1Ees were crossed to a cre-expressing line to facilitate excision of the neomycin resistance cassette. The resulting chimeric protein is composed of amino acids 1-24 followed by 2 tandem repeats of the FLAG tag and the entire C-terminus (amino acids 136-316). RNase protection assay and Western blot analysis confirmed that gene expression and steady-state accumulation of the protein in homozygous mutant animals is similar to that of the wild-type protein. (J:77714)

Expression

In Mice Carrying this Mutation:	4 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tbp Mutation: 10 strains or lines available

References

• Original: J:77714 Hobbs NK, et al., Removing the Vertebrate-Specific TBP N Terminus Disrupts Placental beta2m-Dependent Interactions with the Maternal Immune System. Cell. 2002 Jul 12;110(1):43-54
• All: 3 reference(s)