Tpst1^tm1Klm
Targeted Allele Detail

Summary

• Symbol: Tpst1^tm1Klm
• Name: protein-tyrosine sulfotransferase 1; targeted mutation 1, Kevin L Moore
• MGI ID: MGI:2183366
• Synonyms: Tpst^-
• Gene: Tpst1 Location: Chr5:130102167-130164570 bp, + strand Genetic Position: Chr5, 68.53 cM, cytoband F-G1
• Alliance: Tpst1^tm1Klm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77674
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 3, the first coding exon, was replaced with a neomycin selection cassette. A truncated transcript was detected in RNA derived from the testes and spleens of mutant mice. This transcript was undetected using a probe for exon 3, indicating that sequence encoding the 5' and 3' phosphate-binding domains was absent, thus translation would not result in a functional protein. (J:77674)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tpst1 Mutation: 30 strains or lines available

References

• Original: J:77674 Ouyang YB, et al., Reduced body weight and increased postimplantation fetal death in tyrosylprotein sulfotransferase-1-deficient mice. J Biol Chem. 2002 Jun 28;277(26):23781-7
• All: 8 reference(s)