Rbp3^tm1Gil
Targeted Allele Detail

Summary

• Symbol: Rbp3^tm1Gil
• Name: retinol binding protein 3, interstitial; targeted mutation 1, Gregory I Liou
• MGI ID: MGI:2183368
• Synonyms: IRBP-
• Gene: Rbp3 Location: Chr14:33675960-33686173 bp, + strand Genetic Position: Chr14, 20.8 cM
• Alliance: Rbp3^tm1Gil page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77624
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Approximately 4.4kb of sequence was replaced by a PGK-neo selection cassette. The deleted region contained the promoter and all but 24 base pairs of exon 1. While transcript was undetected by RT-PCR analysis in homozygous mutant mice, Northern blot analysis of total retinal RNA showed the presence of a truncated transcript containing the PGK-neo transgene and the untargeted exons. Immunocytochemistry showed the absence of protein in homozygous mutant mice. Western blot analysis confirmed the staining results as well as verified the lack of protein produced from the truncated transcript. (J:77624)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rbp3 Mutation: 90 strains or lines available

References

• Original: J:77624 Liou GI, et al., Early onset photoreceptor abnormalities induced by targeted disruption of the interphotoreceptor retinoid-binding protein gene. J Neurosci. 1998 Jun 15;18(12):4511-20
• All: 26 reference(s)