Dad1^tm1Wnt
Targeted Allele Detail

Summary

• Symbol: Dad1^tm1Wnt
• Name: defender against cell death 1; targeted mutation 1, Astar Winoto
• MGI ID: MGI:2183460
• Synonyms: Ndelta26
• Gene: Dad1 Location: Chr14:54472942-54491386 bp, - strand Genetic Position: Chr14, 27.7 cM
• Alliance: Dad1^tm1Wnt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77676
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Dad1^tm1Wnt involves 1 genes/genome features (Rr312) View all
• Mutation details: The Dad1 locus was disrupted by the insertion of a neomycin selection cassette that replaced non-coding exon 3 of Dad1 and the Tcra associated LCR (DNAse I hypersensitive sites 2 through 6; Rr312). Neither Dad1 transcript nor protein was detected in homozygous mutant mice by RNAse protection or Western blot analyses. Expression analysis showed the deleted hypersensitive sites were necessary for the enhancement of Tcra transcription in a fraction of immature T cells. (J:77517, J:77676)

Expression

In Mice Carrying this Mutation:

12 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dad1 Mutation: 9 strains or lines available

References

• Original: J:77676 Hong NA, et al., A targeted mutation at the T-cell receptor alpha/delta locus impairs T-cell development and reveals the presence of the nearby antiapoptosis gene Dad1. Mol Cell Biol. 1997 Apr;17(4):2151-7
• All: 2 reference(s)