Nthl1^tm1Yas
Targeted Allele Detail

Summary

• Symbol: Nthl1^tm1Yas
• Name: nth (endonuclease III)-like 1 (E.coli); targeted mutation 1, Akira Yasui
• MGI ID: MGI:2183467
• Synonyms: mNth1 -, Nth1^-
• Gene: Nthl1 Location: Chr17:24851656-24857812 bp, + strand Genetic Position: Chr17, 12.42 cM, cytoband A3
• Alliance: Nthl1^tm1Yas page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77767
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 3 and 4, encoding the conserved helix-hairpin-helix domain, were deleted by the insertion of a neomycin selection cassette. The absence of full-length transcript was verifed via RT-PCR analysis. The expression of the adjacent Tsc2 was unaffected. Sequence analysis revealed that should an aberrantly spliced transcript arise via readthrough of the neo transgene, the splicing of exon 2 to exon 5 would generate a frameshift mutation that precludes production of a functional protein. (J:77767)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nthl1 Mutation: 12 strains or lines available

References

• Original: J:77767 Takao M, et al., Novel nuclear and mitochondrial glycosylases revealed by disruption of the mouse Nth1 gene encoding an endonuclease III homolog for repair of thymine glycols. EMBO J. 2002 Jul 1;21(13):3486-93
• All: 3 reference(s)