Hnf4a^tm1.2Gonz
Targeted Allele Detail

Summary

• Symbol: Hnf4a^tm1.2Gonz
• Name: hepatic nuclear factor 4, alpha; targeted mutation 1.2, Frank J Gonzalez
• MGI ID: MGI:2183525
• Synonyms: Hnf4-alpha^-
• Gene: Hnf4a Location: Chr2:163348731-163414827 bp, + strand Genetic Position: Chr2, 84.32 cM
• Alliance: Hnf4a^tm1.2Gonz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:67396
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated, along with Hnf4^tm1.1Gonz, by breeding mice carrying Hnf4^tm1Gonz with transgenic mice expressing cre recombinase via the EIIa promoter. Expression of cre resulted in the excision of exons 4 and 5 as well as the floxed neomycin cassette. Northern blot and RT-PCR analyses identified a truncated transcript. Sequence analysis showed that the deletion intoduced a frameshift mutation via the splicing of exons 3 and 6. Western blot analysis confirmed the presence of a shortened peptide, putatively lacking several functional domains, including the A and T boxes, the ligand-binding domain, and the activation function 2 domain. (J:67396)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hnf4a Mutation: 28 strains or lines available

References

• Original: J:67396 Hayhurst GP, et al., Hepatocyte nuclear factor 4alpha (nuclear receptor 2A1) is essential for maintenance of hepatic gene expression and lipid homeostasis. Mol Cell Biol. 2001 Feb;21(4):1393-403
• All: 6 reference(s)