Agtr1a^tm2Tma
Targeted Allele Detail

Summary

• Symbol: Agtr1a^tm2Tma
• Name: angiotensin II receptor, type 1a; targeted mutation 2, Taiji Matsusaka
• MGI ID: MGI:2183543
• Synonyms: Agtr1a-, Agtr1a^gfp
• Gene: Agtr1a Location: Chr13:30520424-30566850 bp, + strand Genetic Position: Chr13, 13.19 cM
• Alliance: Agtr1a^tm2Tma page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:36090
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 190 bp of the gene coding region was replaced with a gfp-PGK-neo cassette via homologous recombination. The gfp-PGK-neo cassette was inserted in frame after the third transmembrane domain. Northern blot analysis of kidney tissue confirmed the absence of gene expression in homozygous mutant animals. Although gfp transcripts were present in the kidneys of mutant animals, gfp activity could not be detected. (J:36090)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Agtr1a Mutation: 40 strains or lines available

References

• Original: J:36090 Matsusaka T, et al., Chimeric mice carrying 'regional' targeted deletion of the angiotensin type 1A receptor gene. Evidence against the role for local angiotensin in the in vivo feedback regulation of renin synthesis in juxtaglomerular cells. J Clin Invest. 1996 Oct 15;98(8):1867-77
• All: 3 reference(s)