Del(8Pcid2-Cul4a)1Ktc
Targeted Allele Detail

Summary

• Symbol: Del(8Pcid2-Cul4a)1Ktc
• Name: deletion, Chr 8, Kristin T Chun 1
• MGI ID: MGI:2183544
• Synonyms: CUL-4A-delta1, Cul4a/Pcid2^tm1Ktc
• Gene: Del(8Pcid2-Cul4a)1Ktc Location: unknown Genetic Position: Chr8, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:78032
• Parent Cell Line: CCE916 (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intergenic deletion, Intragenic deletion
• Del(8Pcid2-Cul4a)1Ktc involves 2 genes/genome features (Pcid2, Cul4a) View all
• Mutation details: A floxed region containing exon 1 and approximately 1.1 kb of upstream sequence was deleted by the expression of cre recombinase in vitro. Though sequence analysis showed the deletion to generate an in frame translational start codon at position 146, a truncated protein product was undetected by Western blot analysis of homozygous mutant mice. In a subsequent publication (J:150427), the authors demonstrate that that the upstream loxP site was inserted in a separate gene, Pcid2, that lies in a head-to-head configuration with Cul4a. This loxP site, inserted in a NotI restriction site, lies downstream of the putative translational start site of the gene. Thus the cre-mediated excision potentially disrupted both genes. RNAi knockdown of the Pcid2 transcript in ES cells suggests the embryonic lethality associated with this allele is at least partially due to disruption of the Pcid2 gene. (J:78032, J:150427)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Del(8Pcid2-Cul4a)1Ktc Mutation: 0 strains or lines available

References

• Original: J:78032 Li B, et al., CUL-4A is critical for early embryonic development. Mol Cell Biol. 2002 Jul;22(14):4997-5005
• All: 5 reference(s)