Ehmt2^tm1Yshk
Targeted Allele Detail

Summary

• Symbol: Ehmt2^tm1Yshk
• Name: euchromatic histone lysine N-methyltransferase 2; targeted mutation 1, Yoichi Shinkai
• MGI ID: MGI:2183571
• Synonyms: G9a^-
• Gene: Ehmt2 Location: Chr17:35117445-35133028 bp, + strand Genetic Position: Chr17, 18.45 cM
• Alliance: Ehmt2^tm1Yshk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77998
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The insertion of neomycin selection cassette deleted sequence encoding the glutamic acid stretch, adjacent cysteine-rich region, and ankyrin repeats. While low levels of truncated transcript were detected via Northern blot analysis using a probe for the SET domain, neither full-length nor truncated protein was detected by Western blot analysis. (J:77998)

Expression

In Mice Carrying this Mutation:

6 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ehmt2 Mutation: 57 strains or lines available

References

• Original: J:77998 Tachibana M, et al., G9a histone methyltransferase plays a dominant role in euchromatic histone H3 lysine 9 methylation and is essential for early embryogenesis. Genes Dev. 2002 Jul 15;16(14):1779-91
• All: 7 reference(s)