Gna12^tm1Citb
Targeted Allele Detail

Summary

• Symbol: Gna12^tm1Citb
• Name: guanine nucleotide binding protein, alpha 12; targeted mutation 1, California Institute of Technology - Biology
• MGI ID: MGI:2183606
• Synonyms: Galpha12-, Gna12-
• Gene: Gna12 Location: Chr5:140745699-140816186 bp, - strand Genetic Position: Chr5, 79.3 cM
• Alliance: Gna12^tm1Citb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77728
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Approximately one-third of the coding sequence was replaced with a neomycin selection cassette into exon 4. A trunctated transcript was identified in homozygous mutant mice by Northern blot and RT-PCR analyses. Western blot analysis, using antibodies directed against the amino and carboxy termini, showed the absence of protein. (J:77728)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gna12 Mutation: 23 strains or lines available

References

• Original: J:77728 Gu JL, et al., Interaction of G alpha(12) with G alpha(13) and G alpha(q) signaling pathways. Proc Natl Acad Sci U S A. 2002 Jul 9;99(14):9352-7
• All: 38 reference(s)