Itgb4^tm1Efu
Targeted Allele Detail

Summary

• Symbol: Itgb4^tm1Efu
• Name: integrin beta 4; targeted mutation 1, Elaine Fuchs
• MGI ID: MGI:2183630
• Synonyms: beta4-
• Gene: Itgb4 Location: Chr11:115865556-115899238 bp, + strand Genetic Position: Chr11, 80.91 cM
• Alliance: Itgb4^tm1Efu page

Separation at the mesenchymal-epithelial junctions in skin of Itgb4^tm1Efu/Itgb4^tm1Efu mice

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77644
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The insertion of a neomycin selection cassette deleted sequence extending from 115 base pairs downstream of the start codon through intron 3. The absence of normal transcript in homozygous mutant mice was determined via RT-PCR analysis. While truncated transcript was detected, immunoblot analysis showed an absence of protein. (J:77644)

Disease models

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Itgb4 Mutation: 107 strains or lines available

References

• Original: J:77644 Dowling J, et al., Beta4 integrin is required for hemidesmosome formation, cell adhesion and cell survival. J Cell Biol. 1996 Jul;134(2):559-72
• All: 2 reference(s)