Wt1^tm2Asc
Targeted Allele Detail

Summary

• Symbol: Wt1^tm2Asc
• Name: WT1 transcription factor; targeted mutation 2, Andreas Schedl
• MGI ID: MGI:2183642
• Synonyms: KTS
• Gene: Wt1 Location: Chr2:104956874-105003959 bp, + strand Genetic Position: Chr2, 55.06 cM, cytoband E
• Alliance: Wt1^tm2Asc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:71149
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Humanized sequence, Modified isoform(s))
• Mutations: Insertion, Single point mutation
• Mutation details: A GT to GG mutation at the first splice donor site of exon 9 and a floxed neo cassette in intron 9 were introduced via homologous recombination. The point mutation results in the absence of the alternative splice product with the short version of exon 9 skipping the codons at the end of the exon for the 3 amino acids KTS between zinc fingers 3 and 4 called the KTS region: only transcripts with the long version of exon 9, coding for KTS, was expressed. The neo cassette was removed by transient expression of cre recombinase in correctly targeted ES cells. RT-PCR analysis using primers to exons 9 and 10 demonstrated the presence of only +KTS transcripts in homozygous mutant animals. The -KTS transcript is completely absent. (J:71149)

Expression

In Mice Carrying this Mutation:	38 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Wt1 Mutation: 34 strains or lines available

References

• Original: J:71149 Hammes A, et al., Two splice variants of the Wilms' tumor 1 gene have distinct functions during sex determination and nephron formation. Cell. 2001 Aug 10;106(3):319-29
• All: 6 reference(s)