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Wt1tm2Asc
Targeted Allele Detail
Summary
Symbol: Wt1tm2Asc
Name: WT1 transcription factor; targeted mutation 2, Andreas Schedl
MGI ID: MGI:2183642
Synonyms: KTS
Gene: Wt1  Location: Chr2:104956874-105003959 bp, + strand  Genetic Position: Chr2, 55.06 cM, cytoband E
Alliance: Wt1tm2Asc page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:71149
Parent Cell Line:  AB1 (ES Cell)
Strain of Origin:  129S7/SvEvBrd-Hprt1+
Mutation
description
Allele Type:    Targeted (Humanized sequence, Modified isoform(s))
Mutations:    Insertion, Single point mutation
 
Mutation detailsA GT to GG mutation at the first splice donor site of exon 9 and a floxed neo cassette in intron 9 were introduced via homologous recombination. The point mutation results in the absence of the alternative splice product with the short version of exon 9 skipping the codons at the end of the exon for the 3 amino acids KTS between zinc fingers 3 and 4 called the KTS region: only transcripts with the long version of exon 9, coding for KTS, was expressed. The neo cassette was removed by transient expression of cre recombinase in correctly targeted ES cells. RT-PCR analysis using primers to exons 9 and 10 demonstrated the presence of only +KTS transcripts in homozygous mutant animals. The -KTS transcript is completely absent. (J:71149)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 38 assay results
2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation: 1 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Wt1 Mutation:  34 strains or lines available
References
Original:  J:71149 Hammes A, et al., Two splice variants of the Wilms' tumor 1 gene have distinct functions during sex determination and nephron formation. Cell. 2001 Aug 10;106(3):319-29
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/22/2024
MGI 6.24
The Jackson Laboratory