Sp4^tm1Sus
Targeted Allele Detail

Summary

• Symbol: Sp4^tm1Sus
• Name: trans-acting transcription factor 4; targeted mutation 1, Guntram Suske
• MGI ID: MGI:2183651
• Synonyms: Sp4-, Sp4^IRES-lacZ, Sp4^null
• Gene: Sp4 Location: Chr12:118198668-118265175 bp, - strand Genetic Position: Chr12, 63.48 cM
• Alliance: Sp4^tm1Sus page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77894
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2 and 3, encoding the amino terminal activation domains (residues 4 through 557), were replaced with a cassette containing IRES-lacZ and neo genes. Northern blot analysis of total RNA obtained from brain and heart tissue showed an absence of transcript in homozygous mutant mice. A lack of encoded protein was determined by Western blot analysis using an antibody directed against the carboxy terminal. Expression of beta-galactosidase was controlled by the promoter of the endogenous gene. (J:77894)

Expression

In Mice Carrying this Mutation:	16 assay results
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sp4 Mutation: 35 strains or lines available

References

• Original: J:77894 Gollner H, et al., Complex phenotype of mice homozygous for a null mutation in the Sp4 transcription factor gene. Genes Cells. 2001 Aug;6(8):689-97
• All: 4 reference(s)