Cyct^tm1Jlm
Targeted Allele Detail

Summary

• Symbol: Cyct^tm1Jlm
• Name: cytochrome c, testis; targeted mutation 1, Jose Luis Millan
• MGI ID: MGI:2183657
• Synonyms: Cyt ct^-
• Gene: Cyct Location: Chr2:76184286-76190794 bp, - strand Genetic Position: Chr2, 44.94 cM
• Alliance: Cyct^tm1Jlm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77908
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The insertion of a neomycin selection cassette deleted a portion of the first coding exon, including sequence encoding the heme binding site. Northern blot analysis of total testicular RNA showed an absence of transcript in homozygous mutant mice. Encoded protein was undetected by Western blot anlaysis and immunohistochemical staining. Expression analysis showed that the homologous Cycs locus was unaffected by the targeting event. (J:77908)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cyct Mutation: 7 strains or lines available

References

• Original: J:77908 Narisawa S, et al., Testis-specific cytochrome c-null mice produce functional sperm but undergo early testicular atrophy. Mol Cell Biol. 2002 Aug;22(15):5554-62
• All: 4 reference(s)