Sftpa1^tm1Kor
Targeted Allele Detail

Summary

• Symbol: Sftpa1^tm1Kor
• Name: surfactant associated protein A1; targeted mutation 1, Thomas R Korfhagen
• MGI ID: MGI:2183737
• Synonyms: SP-A(-)
• Gene: Sftpa1 Location: Chr14:40853745-40858330 bp, + strand Genetic Position: Chr14, 22.36 cM
• Alliance: Sftpa1^tm1Kor page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:35250
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Portions of exons 3 and 4 (base pairs 795 through 1049) were deleted by the insertion of a PGK-neo cassette. Northern blot analysis of RNA obtained from lung tissue showed an absence of transcript in homozygous mutant mice. The lack of encoded protein was confirmed by Western blot analysis of lung homogenates and immunohistochemical staining of alveolar type II cells. (J:35250)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sftpa1 Mutation: 21 strains or lines available

References

• Original: J:35250 Korfhagen TR, et al., Altered surfactant function and structure in SP-A gene targeted mice. Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9594-9
• All: 32 reference(s)