Kif3c^tm1Gsn
Targeted Allele Detail

Summary

• Symbol: Kif3c^tm1Gsn
• Name: kinesin family member 3C; targeted mutation 1, Lawrence S B Goldstein
• MGI ID: MGI:2183787
• Synonyms: Kif3c^typeI
• Gene: Kif3c Location: Chr12:3415132-3456494 bp, + strand Genetic Position: Chr12, 1.77 cM
• Alliance: Kif3c^tm1Gsn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:70607
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Two loxP sites were introduced to the intron and 5' untranslated region flanking exon 1, and a third loxP site was introduced with a PGK-TK/PGK-neo cassette directly downstream exon 1 via homologous recombination. Exon 1 and the PGK-TK/PGK-neo cassette were removed by transient expression of cre recombinase in correctly targeted ES cells resulting in the deletion of 2.1 kb of DNA encoding amino acids 1-518 which include the whole motor region and half of the alpha-helical coiled coil domains. Northern blot analysis detected a truncated transcript encoding the tail end of the gene in homozygous mutant animals. Truncated protein product was detected in homozygous mutants by Western blot analysis using antibodies recognizing the tail end of the protein. Immunoprecipitation of brain lysates from homozygous mutants demonstrated the ability of the truncated protein to specifically bind Kif3a and not Kif3b. (J:70607)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Kif3c Mutation: 24 strains or lines available

References

• Original: J:70607 Yang Z, et al., Functional analysis of mouse kinesin motor kif3c. Mol Cell Biol. 2001 Aug;21(16):5306-11
• All: 1 reference(s)