Lefty2^tm2Hmd
Targeted Allele Detail

Summary

• Symbol: Lefty2^tm2Hmd
• Name: left-right determination factor 2; targeted mutation 2, Hiroshi Hamada
• MGI ID: MGI:2183818
• Synonyms: lefty2^deltaASE, Rr179^tm2Hmd
• Gene: Lefty2 Location: Chr1:180720673-180726668 bp, + strand Genetic Position: Chr1, 84.46 cM
• Alliance: Lefty2^tm2Hmd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:70483
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 0.5 kb region of the gene containing the asymmetric enhancer (ASE) was replaced with a loxP site flanked neomycin resistance gene cassette via homologous recombination. The floxed neo cassette was subsequently removed by crossing chimeric animals to a cre-expressing line. Ablation of asymmetric gene expression in homozygous mutant embryos was confirmed by whole mount in situ hybridization. (J:70483)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Lefty2 Mutation: 14 strains or lines available

References

• Original: J:70483 Meno C, et al., Diffusion of nodal signaling activity in the absence of the feedback inhibitor Lefty2. Dev Cell. 2001 Jul;1(1):127-138
• All: 2 reference(s)