Eif2ak3^tm1Drc
Targeted Allele Detail

Summary

• Symbol: Eif2ak3^tm1Drc
• Name: eukaryotic translation initiation factor 2 alpha kinase 3; targeted mutation 1, Douglas R Cavener
• MGI ID: MGI:2183829
• Synonyms: Perk^-
• Gene: Eif2ak3 Location: Chr6:70821499-70882229 bp, + strand Genetic Position: Chr6, 32.09 cM
• Alliance: Eif2ak3^tm1Drc page

Acinar cells are lost in both Eif2ak3^tm1Drc/Eif2ak3^tm1Drc and Eif2ak3^tm1.1Drc/Eif2ak3^tm1.1Drc Tg(Ela1-cre)16Eps/0 mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76661
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Two loxP sites were introduced to intronic regions flanking the three exons encoding part of the lumenal domain, the transmembrane domain, and part of the catalytic domain. A third loxP site was introduced with a PGK-neo cassette directly downstream of the second loxP site in the same intron. The three exons and PGK-neo cassette were excised by mating chimeric animals to a cre-expressing line. RT-PCR analysis of homozygous mutant animals using primers flanking the deleted region confirmed that gene transcripts did not contain the deleted exons. Western blot analysis using antibodies directed against the kinase domain did not detect protein product in pancreatic lysates from homozygous mutants. (J:76661)

Disease models

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	13 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Eif2ak3 Mutation: 53 strains or lines available

References

• Original: J:76661 Zhang P, et al., The PERK eukaryotic initiation factor 2 alpha kinase is required for the development of the skeletal system, postnatal growth, and the function and viability of the pancreas. Mol Cell Biol. 2002 Jun;22(11):3864-74
• All: 12 reference(s)