Myh4^tm1Lnwd
Targeted Allele Detail

Summary

• Symbol: Myh4^tm1Lnwd
• Name: myosin, heavy polypeptide 4, skeletal muscle; targeted mutation 1, Leslie A Leinwand
• MGI ID: MGI:2183830
• Synonyms: IIb-
• Gene: Myh4 Location: Chr11:67128855-67151272 bp, + strand Genetic Position: Chr11, 40.59 cM
• Alliance: Myh4^tm1Lnwd page

Muscle abnormalities, such as increased muscle fiber size and interstitial fibrosis, in Myh1^tm1Lnwd/Myh1^tm1Lnwd and Myh4^tm1Lnwd/Myh4^tm1Lnwd mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:44448
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Exon 3, which is the first coding exon, was disrupted by the insertion of a neomycin selection cassette. Low levels of transcript were detected in homozygous mutant mice by RNAse protection assays, however, Western blot analysis showed an absence of encoded protein. (J:44448)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Myh4 Mutation: 94 strains or lines available

References

• Original: J:44448 Acakpo-Satchivi LJ, et al., Growth and muscle defects in mice lacking adult myosin heavy chain genes. J Cell Biol. 1997 Dec 1;139(5):1219-29
• All: 4 reference(s)