Nrp2^tm1.2Mom
Targeted Allele Detail

Summary

• Symbol: Nrp2^tm1.2Mom
• Name: neuropilin 2; targeted mutation 1.2, Peter Mombaerts
• MGI ID: MGI:2183904
• Synonyms: Neuropilin-2-Delta, NP2-delta, Nrp2^del, Nrp2^taugfp, Nrp2^tm1Mom
• Gene: Nrp2 Location: Chr1:62742476-62857851 bp, + strand Genetic Position: Chr1, 31.35 cM, cytoband C2
• Alliance: Nrp2^tm1.2Mom page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76686
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site 272 bp upstream of the translation initiation codon was introduced into exon 1 and a loxP-tauGFP FRT-flanked neo cassette was inserted 1 kb into intron 1 via homologous recombination. Heterozygous animals were crossed to an flp recombinase transgenic line for removal of the neo cassette. Subsequent mating to a cre recombinase transgenic line removed exon 1 and part of intron 1 leaving the tauGFP cassette intact. The exon 1 deletion removes the plasma membrane-targeting signal sequence and also brings the tauGFP cassette under the control of the endogenous promoter. RT-PCR analysis confirmed the removal of the signal sequence in gene transcripts from homozygous mutant animals. Immunohistochemistry of brain sections from homozygous mutants did not detect protein product. (J:76686)

Disease models

Expression

In Mice Carrying this Mutation:	45 assay results
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nrp2 Mutation: 89 strains or lines available

References

• Original: J:76686 Walz A, et al., Aberrant sensory innervation of the olfactory bulb in neuropilin-2 mutant mice. J Neurosci. 2002 May 15;22(10):4025-35
• All: 16 reference(s)